Host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of IL-12 or IFN-α/β
Identifieur interne : 001838 ( Main/Exploration ); précédent : 001837; suivant : 001839Host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of IL-12 or IFN-α/β
Auteurs : Marel C. De Wit [Pays-Bas] ; Marian C. Horzinek [Pays-Bas] ; Bart L. Haagmans [Pays-Bas] ; Virqil E. J. C. Schijns [Pays-Bas]Source :
- Journal of general virology [ 0022-1317 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
Replicating viruses generally induce type 1 immune responses, with high interferon (IFN)-; levels and antibodies of the IgG2a isotype. In the present study we demonstrate the intrinsic ability of non-replicating virions to induce comparable immune responses in the notable absence of any adjuvant. Injection of inactivated pseudorabies virus, an alphaherpesvirus, by various routes into mice resulted in the generation of T helper (Th) 1 type immune response. Co-delivery of inactivated pseudorabies herpesvirus (iPRV) with protein redirected IgG1-dominated tetanus toxoid-specific responses towards an IgG1/IgG2a balanced response. Also inactivated preparations of viruses from the paramyxo- (Newcastle disease virus), rhabdo- (rabies virus), corona- (infectious bronchitis virus) and reovirus (avian reovirus) families led to IgG2a antibody responses; however, the genetic background of the host did result in considerable variation. Because disrupted virions also induced type 1 immune responses, we conclude that structural elements of virions inherently contribute to IFN-γ-dependent isotype switching by inactivated viruses. Strikingly, immunizations in gene-disrupted mice showed that a functional IFN-α/β, IFN-γ or interleukin (IL)-12 pathway was not required for the generation of a polarized Th1 type immune response initiated by inactivated virus particles. These findings have a bearing on the understanding of immune responsiveness to virus structures and the design of vaccines containing virus components.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000055
- to stream PascalFrancis, to step Curation: 000004
- to stream PascalFrancis, to step Checkpoint: 000051
- to stream Main, to step Merge: 001849
- to stream Main, to step Curation: 001838
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of IL-12 or IFN-α/β</title><author><name sortKey="De Wit, Marel C" sort="De Wit, Marel C" uniqKey="De Wit M" first="Marel C." last="De Wit">Marel C. De Wit</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University</s1><s2>3584 CL Utrecht</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Horzinek, Marian C" sort="Horzinek, Marian C" uniqKey="Horzinek M" first="Marian C." last="Horzinek">Marian C. Horzinek</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University</s1><s2>3584 CL Utrecht</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L." last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Virology, Erasmus MC Rotterdam</s1><s2>3015 GE Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>3015 GE Rotterdam</wicri:noRegion></affiliation></author><author><name sortKey="Schijns, Virqil E J C" sort="Schijns, Virqil E J C" uniqKey="Schijns V" first="Virqil E. J. C." last="Schijns">Virqil E. J. C. Schijns</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Vaccine Technology and Immunology, Intervet International BV</s1><s2>5830 AA Boxmeer</s2><s3>NLD</s3><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>5830 AA Boxmeer</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0302805</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0302805 INIST</idno><idno type="RBID">Pascal:04-0302805</idno><idno type="wicri:Area/PascalFrancis/Corpus">000055</idno><idno type="wicri:Area/PascalFrancis/Curation">000004</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000051</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000051</idno><idno type="wicri:doubleKey">0022-1317:2004:De Wit M:host:dependent:type</idno><idno type="wicri:Area/Main/Merge">001849</idno><idno type="wicri:Area/Main/Curation">001838</idno><idno type="wicri:Area/Main/Exploration">001838</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of IL-12 or IFN-α/β</title><author><name sortKey="De Wit, Marel C" sort="De Wit, Marel C" uniqKey="De Wit M" first="Marel C." last="De Wit">Marel C. De Wit</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University</s1><s2>3584 CL Utrecht</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Horzinek, Marian C" sort="Horzinek, Marian C" uniqKey="Horzinek M" first="Marian C." last="Horzinek">Marian C. Horzinek</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University</s1><s2>3584 CL Utrecht</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L." last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Virology, Erasmus MC Rotterdam</s1><s2>3015 GE Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>3015 GE Rotterdam</wicri:noRegion></affiliation></author><author><name sortKey="Schijns, Virqil E J C" sort="Schijns, Virqil E J C" uniqKey="Schijns V" first="Virqil E. J. C." last="Schijns">Virqil E. J. C. Schijns</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Vaccine Technology and Immunology, Intervet International BV</s1><s2>5830 AA Boxmeer</s2><s3>NLD</s3><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>5830 AA Boxmeer</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alpha interferon</term><term>Beta interferon</term><term>Cytokine</term><term>Interleukin 12</term><term>Microbiology</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cytokine</term><term>Interleukine 12</term><term>Interféron bêta</term><term>Interféron alpha</term><term>Microbiologie</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Replicating viruses generally induce type 1 immune responses, with high interferon (IFN)-; levels and antibodies of the IgG2a isotype. In the present study we demonstrate the intrinsic ability of non-replicating virions to induce comparable immune responses in the notable absence of any adjuvant. Injection of inactivated pseudorabies virus, an alphaherpesvirus, by various routes into mice resulted in the generation of T helper (Th) 1 type immune response. Co-delivery of inactivated pseudorabies herpesvirus (iPRV) with protein redirected IgG1-dominated tetanus toxoid-specific responses towards an IgG1/IgG2a balanced response. Also inactivated preparations of viruses from the paramyxo- (Newcastle disease virus), rhabdo- (rabies virus), corona- (infectious bronchitis virus) and reovirus (avian reovirus) families led to IgG2a antibody responses; however, the genetic background of the host did result in considerable variation. Because disrupted virions also induced type 1 immune responses, we conclude that structural elements of virions inherently contribute to IFN-γ-dependent isotype switching by inactivated viruses. Strikingly, immunizations in gene-disrupted mice showed that a functional IFN-α/β, IFN-γ or interleukin (IL)-12 pathway was not required for the generation of a polarized Th1 type immune response initiated by inactivated virus particles. These findings have a bearing on the understanding of immune responsiveness to virus structures and the design of vaccines containing virus components.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Utrecht (province)</li></region><settlement><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="De Wit, Marel C" sort="De Wit, Marel C" uniqKey="De Wit M" first="Marel C." last="De Wit">Marel C. De Wit</name></region><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L." last="Haagmans">Bart L. Haagmans</name><name sortKey="Horzinek, Marian C" sort="Horzinek, Marian C" uniqKey="Horzinek M" first="Marian C." last="Horzinek">Marian C. Horzinek</name><name sortKey="Schijns, Virqil E J C" sort="Schijns, Virqil E J C" uniqKey="Schijns V" first="Virqil E. J. C." last="Schijns">Virqil E. J. C. Schijns</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001838 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001838 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:04-0302805
|texte= Host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of IL-12 or IFN-α/β
}}
| This area was generated with Dilib version V0.6.33. |  |